# System preparation A focused tutorial set on preparing biomolecular systems for simulation: protonation, non-standard residues, custom residues from SMILES, and mutation/gap-closing/segmentation. ```{toctree} :maxdepth: 1 01-basic-protonation 02-non-standard-residues 03-custom-residues-from-smiles 04-mutation-gap-closing-segmentation ```